Duct Duct Goose 4

Duct Duct Goose 4

Dexter X. Jin, Daniel H. Miller

MIT Department of Biology, Whitehead Institute, Koch Institute at MIT

We blocked specific genes from functioning in human breast tissue while growing them into mini-organs in our 3D growth system. We then fluorescently marked them for differentiation states.

Chemical perturbations to specific genes in our mini-organ culture system resulted in marked morphological changes from the normal mammary mini-organ. We sought to characterize these changes by following markers of differentiation after chemical treatments.

By synergizing our three-dimensional patient derived mammary mini-organ system with chemical perturbations, we can functionally dissect the roles of genes during mammary development; for example, during stem cell differentiation.

See the winning image from this project here.

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