A Zebrafish Model of Uveal Melanoma 2

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Submitted by Dahlia Perez of the Lees Laboratory at the Koch Institute for Integrative Cancer Research at MIT

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Though humans and fish may seem incredibly different, the zebrafish stripes contain pigmented cells analogous to human melanocytes, melanin containing cells found in eye and skin. Because of these similarities, we were able to construct a zebrafish model of uveal melanoma, a highly metastatic human cancer arising in the eye.

This picture focuses on the skin of a zebrafish expressing one of two transgenes harboring mutations detected in 85% of uveal melanoma cases. Melanocytes (the darkest cells) are aberrantly present in the interstripes (shiny, yellow stripe). The interstripes themselves are wavy as opposed to straight as in the wild type controls. Correct pigment patterning in zebrafish requires proper stem cell differentiation, cell motility and cell-cell signaling. By documenting and understanding the reasons for the change in pigment pattern in the transgene containing fish, we can elucidate the function of the transgene in these pathways.