Replication Machinery in Bacillus Subtilis

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Submitted by Melanie Berkmen of the Grossman Laboratory in the Department of Biology

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Melanie Berkmen
Grossman Laboratory, Department of Biology
Epi-Fluorescence Micrograph

"This image shows simultaneous localization of the origin of replication and the replication machinery in live Bacillus subtilis cells.  Cell outlines (shown in white/grey) were visualized with the vital membrane stain FM4-64.  The origin region (cyan) was visualized using LacI-CFP bound to lac operators inserted near oriC. The replisome (yellow) was visualized using a fusion of the Tau subunit of DNA polymerase to YFP."