Three Dimensional Patterning of Hepatocytes and Fibroblasts


Submitted by Kelly Stevens and Sangeeta Bhatia of the Laboratory for Multiscale Regenerative Technologies at MIT


Kelly Stevens and Sangeeta Bhatia
Laboratory for Multiscale Regenerative Technologies, Koch Institute
Epi-Fluorescence Micrograph 

"Human tissues are composed of many cell types that are intricately organized on the micro-scale.  Such organized "architecture" is known to dictate organ function.  We are working to develop engineering technologies that allow us to precisely organize multiple cell types within engineered tissues so that we can 1) study how such organization alters tissue function, and 2) develop improved engineered tissues for translational human therapy.

This image depicts a new three dimensional multi-cellular patterning strategy for engineering three-dimensional tissues.  Rat hepatocytes and fibroblasts (red) are patterned within an endothelial cell lattice (green) with precise spatial control."